Only affects new installations, or when the user presses the 'reset to default colors' button. MKVToolNix GUI: multiplexer: the default list colors used for distinguishing source files does not include the darkest tier anymore (the ones where one or two components are set to the value 0x3f) as they're hard to distinguish from each other.

Note that this doesn't affect file identification. Tracks of the same type will be sorted in the same order as their source files occur in the command-line arguments. The order is as follows: video tracks first followed by audio & subtitle tracks with other rarely used types of tracks last.

mkvmerge: tracks in the destination file will now be sorted by their type automatically unless the track order is specified with the -track-order option.

Removing block additional data is not possible anymore, as it is often required for proper decoding.

mkvmerge: removed the option -blockadd.

You can find more information about it and its underlying technology, the Extensible Binary Meta Language (EBML), at What's New Matroska is a new multimedia file format aiming to become the new container format for the future. With these tools, one can get information about (mkvinfo) Matroska files, extract tracks/data from (mkvextract) Matroska files and create (mkvmerge) Matroska files from other media files.

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